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Sigma
Acid Phosphatase, Leukocyte (TRAP) Kit
387A -
Kit formulated with all liquid reagents
DOWNLOAD MSDS (PDF)Properties
| Related Categories | Cytochemistry Products, Hematology and Histology |
| Quality Level | IVD |
| shelf life | (Expiry date on the label.) |
| IVD | for in vitro diagnostic use |
| storage temp. | 2-8°C |
Description
Frequently Asked Questions
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are available for this Product.
Principle
Peripheral blood or bone marrow preparations are fixed to a microscope slide. The resulting film is incubated in a solution of Naphthol AS-BI phosphoric acid and freshly diazotized Fast Garnet GBC. Kit is used to demonstrate acid phosphatase and tartrate resistant acid phosphatase (TRAP) in blood, bone marrow and tissue touch preparations.
Application
Tartrate resistant acid phosphatase (TRAP) is a glycosylated monomeric metalloenzyme highly expressed in mammals cells such as osteoclasts, activated macrophages, and neurons. TRAP expression is increased in certain pathological conditions, such as reticuloendotheliosis, Gaucher’s disease, HIV-induced encephalopathy, osteoclastoma and and osteoporosis.
Price and Availability
Kit component only
Description |
|---|
| Hematoxylin Solution, Gill No. 3 (kit only) 50 mL |
Kit component also available separately
Description |
|---|
| Acetate Solution (386-3) 50 mL |
| Citrate Solution (91-5) 50 mL |
| Fast Garnet GBC Base Solution (387-2) 10 mL |
| Naphthol AS-BI Phosphoric Acid (387-1) 10 mL |
| Sodium Nitrite Solution (91-4) 10 mL |
| Tartrate Solution (387-3) 10 mL |
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Kit formulated with all liquid reagents.
based on naphthol AS-BI and fast red violet LB
Hanker-Yates substrate kit
Safety Information
| Symbol |    GHS05, GHS07, GHS08 |
| Signal word | Danger |
| Hazard statements | H302-H314-H350 |
| Precautionary statements | P201-P280-P305 + P351 + P338-P310 |
| Hazard Codes | T,Xn,Xi |
| Risk Statements | 36-36/37/38 |
| Safety Statements | 36/37-45 |
| RIDADR | UN 1789 8/PG 2 |
Technical information & documentation associated with this product is available in the Safety & Documentation tab.
References
Oshita K, Yamaoka K, et. al., Human mesenchymal stem cells inhibit osteoclastogenesis through osteoprotegerin production. Arthritis Rheum., (2011)
Stempel J, Fritsch H, Pfaller K, Blumer MJ., Development of articular cartilage and the metaphyseal growth plate: the localization of TRAP cells, VEGF, and endostatin. J. Anat. 218, 608-618, (2011) Abstract
Lausch E, Janecke A, Bros M, Trojandt S, et. al., Genetic deficiency of tartrate-resistant acid phosphatase associated with skeletal dysplasia, cerebral calcifications and autoimmunity. Nat. Genet. 43, 132-137, (2011) Abstract
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